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MicroRNA-210 Regulates Endoplasmic Reticulum Stress and Apoptosis in Porcine Embryos

Muhammad Rosyid Ridlo1,2, Eui Hyun Kim1, Geon A Kim3

  • 1Department of Theriogenology and Biotechnology, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.

Animals : an Open Access Journal From MDPI
|January 22, 2021
PubMed

Insights

In vitro embryo production is hindered by endoplasmic reticulum (ER) stress. Inhibiting microRNA-210 (miR-210) improves embryo development by reducing ER stress and apoptosis.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Endoplasmic reticulum (ER) stress impedes in vitro embryo production and survival.
  • MicroRNA-210 (miR-210) is implicated in cellular stress adaptation and inflammation.

Purpose of the Study:

  • To investigate the role of miR-210 in porcine in vitro embryo development.
  • To determine the effects of miR-210 on ER stress and apoptosis during early embryonic development.

Main Methods:

  • Microinjection of miR-210 inhibitor or mimic into parthenogenetically activated porcine embryos.
  • Assessment of embryo developmental rates (cleavage and blastocyst formation).
  • Quantification of total cell number (TCN) in blastocysts.
  • Analysis of gene expression related to ER stress (XBP1, uXBP1, sXBP1, ATF4, PTPN1), apoptosis (Caspase 3), and pluripotency (NANOG, SOX2).

Main Results:

  • miR-210 inhibition significantly improved cleavage rates, blastocyst formation, and TCN, while reducing XBP1 expression.
  • Inhibition of miR-210 decreased ER stress markers (uXBP1, sXBP1, ATF4, PTPN1), apoptosis (Caspase 3), and increased pluripotency markers (NANOG, SOX2).
  • Conversely, miR-210 mimic injection impaired embryo development, downregulated ER stress and apoptosis markers, and reduced pluripotency markers.

Conclusions:

  • miR-210 plays a critical role in regulating porcine in vitro embryo development.
  • Inhibiting miR-210 can alleviate ER stress and reduce apoptosis, thereby enhancing in vitro embryo production outcomes.
Keywords:
apoptosisendoplasmic reticulum stressin vitro culturemiR-210-inhibitormiR-210-mimicparthenogenetic activationpig

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