Detection and Quantification of Transforming Growth Factor-β1 Produced by Murine B Cells: Pros and Cons of Different
Yoshiyuki Mishima1, Akihiko Oka2, Shunji Ishihara2
1Department of Internal Medicine II, Shimane University Faculty of Medicine, Izumo, Japan. mtmtyui@med.shimane-u.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|January 22, 2021
Abstract:
Transforming growth factor (TGF)-β1 is one of the regulatory cytokines produced by B cells and has a pivotal role in intestinal homeostasis. TGF-β1 can determine the fate of naive T cells, such as differentiation, proliferation, and apoptosis, which are relevant to the pathogenesis of autoimmunity, infection, inflammation, allergy, and cancer. Here, we describe detailed methods for detection and quantification of TGF-β1 secreted by B cells using ELISA, flow cytometry, and real-time PCR.
