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Quantitative proteomic analysis in symptomatic and asymptomatic apical periodontitis
C Loureiro1, M A R Buzalaf2, F R N Moraes1
1Department of Preventive and Restorative Dentistry, Aracatuba School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
International Endodontic Journal
|January 22, 2021
Summary
Symptomatic and asymptomatic apical periodontitis (AP) show distinct host proteomic profiles, with differences in immune response proteins and viral presence markers. These findings enhance understanding of AP
Area of Science:
- Proteomics
- Oral Biology
- Immunology
Background:
- Apical periodontitis (AP) is an inflammatory condition affecting the periapical tissues.
- Understanding the host response in symptomatic versus asymptomatic AP is crucial for diagnosis and treatment.
- Proteomic analysis offers a powerful tool to investigate complex biological processes in disease.
Purpose of the Study:
- To quantitatively and qualitatively compare host proteomic profiles in symptomatic and asymptomatic AP.
- To identify specific proteins associated with each clinical presentation of AP.
- To elucidate the molecular pathways underlying symptomatic and asymptomatic AP.
Main Methods:
- Nano-liquid chromatography-electron spray tandem mass spectrometry was employed for proteomic analysis.
- Samples from 18 patients (9 symptomatic, 9 asymptomatic AP) were analyzed.
- Label-free quantitative proteomic analysis and Monte Carlo statistical methods were utilized.
Main Results:
- A total of 853 human proteins were identified.
- Significant differences in protein expression were observed between symptomatic and asymptomatic groups.
- Symptomatic AP showed up-regulated proteins related to immune response, oxidative stress, and viral presence, while asymptomatic AP had distinct protein profiles.
Conclusions:
- Distinct quantitative and qualitative proteomic profiles exist for symptomatic and asymptomatic AP.
- Proteins identified are primarily associated with host immune responses.
- The findings provide a foundation for understanding AP pathogenesis and developing targeted therapies.
