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Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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High-Performance Liquid Chromatography: Elution Process01:05

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Instrumentation00:57

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Optimizing Chromatographic Separations01:15

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-Performance Liquid Chromatography: Types of Detectors01:15

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Updated: Nov 20, 2025

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example
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Process development and optimization of continuous capture with three-column periodic counter-current chromatography.

Ce Shi1, Qi-Lei Zhang1, Biao Jiao2

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.

Biotechnology and Bioengineering
|January 22, 2021
PubMed
Summary

Model-assisted tools optimize continuous manufacturing of monoclonal antibodies (mAbs) using three-column periodic counter-current chromatography (3C-PCC). This approach enhances process development, achieving high productivity and capacity utilization for efficient mAb production.

Keywords:
continuous chromatographymodel-based approachperiodic counter-current chromatographyprocess optimization

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Area of Science:

  • Biopharmaceutical manufacturing
  • Chemical engineering
  • Process chromatography

Background:

  • Continuous capture using affinity chromatography is crucial for monoclonal antibody (mAb) manufacturing.
  • Three-column periodic counter-current chromatography (3C-PCC) presents development and optimization complexities.

Purpose of the Study:

  • To evaluate and compare experimental, model-based, and simplified approaches for 3C-PCC process development.
  • To investigate the impact of key operating parameters on productivity and capacity utilization in 3C-PCC.

Main Methods:

  • Comparative analysis of experimental, model-based, and simplified methodologies for 3C-PCC optimization.
  • Focus on residence time (RT C), breakthrough percentage (s), and feed concentration (c 0) effects.
  • Model-based approach utilized for predicting operation space and optimizing parameters.

Main Results:

  • The model-based approach demonstrated superiority over the experimental method for process optimization.
  • Two distinct productivity phases were identified, with optimal RT C found at their boundary.
  • Maximized productivity of 34.5 g/L/h and 97.6% capacity utilization achieved with MabSelect SuRe LX resin.

Conclusions:

  • Model-assisted tools are effective for optimizing 3C-PCC continuous capture processes.
  • The study identified optimal operating conditions for high productivity and capacity utilization in mAb manufacturing.
  • A simplified approach was proposed for determining optimal RT C for maximum productivity.