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Updated: Nov 20, 2025

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling
Noah Steinfeld1,2, Sai Srinivas Panapakkam Giridharan1, Emily J Kauffman1
1Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Abstract:
Phosphoinositide (PPI) lipids are a crucial class of low-abundance signaling molecules that regulate many processes within cells. Methods that enable simultaneous detection of all PPI lipid species provide a wholistic snapshot of the PPI profile of cells, which is critical for probing PPI biology. Here we describe a method for the simultaneous measurement of cellular PPI levels by metabolically labeling yeast or mammalian cells with myo-3H-inositol, extracting radiolabeled glycerophosphoinositides, and separating lipid species on an anion exchange column via HPLC.

