Related Experiment Video

Updated: Nov 20, 2025

Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

87.6K

Circulating long non-coding RNAs HOTAIR, Linc-p21, GAS5 and XIST expression profiles in diffuse large B-cell

Mahmoud A Senousy1, Aya M El-Abd2, Raafat R Abdel-Malek3

  • 1Department of Biochemistry, Faculty of Pharmacy, Cairo University, 23 Kasr Al-Ainy street, Cairo, 11562, Egypt.

Scientific Reports
|January 23, 2021
PubMed

Insights

Plasma long non-coding RNAs HOTAIR, GAS5, and XIST show promise as non-invasive biomarkers for diagnosing diffuse large B-cell lymphoma (DLBCL) and predicting response to R-CHOP therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biomarkers

Background:

  • Diffuse large B-cell lymphoma (DLBCL) requires reliable diagnostic and therapeutic targets.
  • Long non-coding RNAs (lncRNAs) are involved in DLBCL pathogenesis but circulating lncRNAs remain understudied.
  • Identifying circulating lncRNAs can improve DLBCL management.

Purpose of the Study:

  • To investigate plasma lncRNAs (HOTAIR, Linc-p21, GAS5, XIST) as biomarkers for DLBCL diagnosis.
  • To assess these lncRNAs' potential in predicting response to R-CHOP therapy.
  • To explore the prognostic value of these lncRNAs in DLBCL patients.

Main Methods:

  • Plasma samples from 84 DLBCL patients and 33 healthy controls were analyzed.
  • Expression levels of HOTAIR, Linc-p21, GAS5, and XIST were quantified.
  • Receiver operating characteristic (ROC) analysis and multivariate analysis were performed to evaluate diagnostic and predictive accuracy.

Main Results:

  • Plasma HOTAIR, XIST, and GAS5 were differentially expressed in DLBCL patients versus controls.
  • Pretreatment HOTAIR was higher in non-responders, while GAS5 was lower compared to responders.
  • Plasma GAS5 showed high diagnostic accuracy (AUC=0.97); HOTAIR+GAS5 panel predicted treatment response; HOTAIR independently predicted non-response.

Conclusions:

  • Plasma HOTAIR, GAS5, and XIST serve as potential non-invasive diagnostic biomarkers for DLBCL.
  • Pretreatment HOTAIR and GAS5 can predict R-CHOP therapy response, with HOTAIR indicating R-CHOP failure.
  • These lncRNAs offer novel surrogates for personalized medicine and predictive studies in DLBCL.

More Related Videos

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.3K
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.3K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.4K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.1K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.0K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.6K