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Related Experiment Videos

Microsphere estimates of blood flow: methodological considerations.

C von Ritter1, R A Hinder, W Womack

  • 1Department of Surgery, University of Witwatersrand, Johannesburg, South Africa.

The American Journal of Physiology
|February 1, 1988
PubMed
Summary

Blood flow estimates using radioactive microspheres in dogs are affected by blood withdrawal. Replacing lost blood volume with dextran 70 ensures stable organ blood flow during the microsphere technique.

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Area of Science:

  • Physiology
  • Cardiovascular Research
  • Experimental Medicine

Background:

  • The microsphere technique is a common method for measuring blood flow in animal models.
  • Previous studies have noted limitations, but a systematic assessment was lacking.

Purpose of the Study:

  • To systematically evaluate factors affecting blood flow estimates using radioactive microspheres in dogs.
  • To assess the impact of reference sample blood withdrawals, microsphere quantity, and anesthesia on blood flow measurements.

Main Methods:

  • Radioactive microspheres (15 microns) were used for blood flow estimates in conscious (18) and anesthetized (12) dogs over 120 minutes.
  • Evaluated effects of excessive microspheres (13 million), pentobarbital sodium anesthesia, and dextran 70 for volume replacement.

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  • Monitored gastric mucosal blood flow, cardiac output, and pancreatic blood flow.

    • Main Results:

    • Progressive decreases in gastric mucosal blood flow and cardiac output were observed over time in both groups.
    • Pancreatic blood flow also decreased in conscious dogs.
    • Volume loss from reference samples was identified as the primary cause of decreased blood flow.
    • Replacing withdrawn blood with dextran 70 stabilized blood flow to all organs.
    • Excessive microsphere numbers did not significantly alter hemodynamics compared to standard amounts.
    • Anesthesia minimally affected blood flow, primarily increasing coronary flow.

    Conclusions:

    • The microsphere technique can lead to decreased organ blood flow due to reference sample volume loss.
    • Replacing withdrawn blood volume with dextran 70 is crucial for maintaining stable blood flow measurements.
    • Anesthesia has minimal impact on overall blood flow dynamics, except for coronary flow.