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Related Concept Videos

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Micro-RNA gene expressions during cardiopulmonary bypass.

Perfusionist Tülay Aydın1, Mustafa Göz1, Nazım Kankılıç1

  • 1Department of Cardiovascular Surgery, Medical School of Harran University, Şanlıurfa, Turkey.

Journal of Cardiac Surgery
|January 23, 2021
PubMed
Summary

Cardiopulmonary bypass (CPB) significantly alters microRNA (miRNA) gene expression. Specifically, miR-34a, miR-15a, and miR-320a levels increase during CPB, suggesting miRNAs role in cardiac surgery complications.

Keywords:
cardiopulmonary bypassmiR-15amiR-320amiR-34a

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Area of Science:

  • Molecular Biology
  • Cardiovascular Surgery

Background:

  • Cardiopulmonary bypass (CPB) is a cornerstone of modern cardiac surgery.
  • Maintaining myocardial perfusion and mitigating oxidative stress are critical during CPB.
  • MicroRNAs (miRNAs) are emerging as key regulators of cellular processes affected by CPB.

Purpose of the Study:

  • To investigate the dynamic changes in specific microRNA (miRNA) gene expression during cardiopulmonary bypass (CPB).
  • To analyze the expression patterns of miR-34a, miR-15a, and miR-320a in patients undergoing cardiac surgery with CPB.

Main Methods:

  • Study included fifteen patients undergoing elective open-heart surgery.
  • Serum plasma samples collected preoperatively, during CPB, and 24 hours postoperatively.
  • Quantitative analysis of miR-34a, miR-15a, and miR-320a gene expression.

Main Results:

  • miR-15a gene expression significantly increased during CPB (p < .001), decreasing postoperatively (p < .05).
  • miR-34a gene expression showed a significant increase during CPB (p < .01).
  • miR-320a gene expression was also significantly elevated during CPB (p < .01).

Conclusions:

  • MicroRNAs (miRNAs) likely play a crucial role in the pathophysiological responses to cardiopulmonary bypass (CPB).
  • This study provides initial evidence for the altered expression of miR-34a, miR-15a, and miR-320a during CPB.
  • Findings pave the way for future research into the molecular mechanisms underlying CPB-associated complications.