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Updated: Nov 20, 2025
Immunofluorescent Detection of Two Thymidine Analogues CldU and IdU in Primary Tissue
Published on: December 7, 2010
Rapid, user-friendly, and inexpensive detection of azidothymidine
Ying Luo1, Tianwei Jia1, Jieqiong Fang1
1788 Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, 161 Jesse Hill Jr. Drive, Atlanta, GA, 30302, USA.
Abstract:
Strict adherence to highly active antiretroviral therapy (HAART) is very important to improve the quality of life for HIV-positive patients to reduce new infections and determine treatment success. Azidothymidine (AZT) is an antiretroviral drug commonly used in HAART treatment. In this research, an "add, mix, and measure" assay was developed to detect AZT within minutes. Three different probes designed to release fluorophores when samples containing AZT are added were synthesized and characterized. The limit of detection to AZT in simulated urine samples was determined to be 4 μM in 5 min for one of the probes. This simple and rapid point-of-care test could potentially be used by clinicians and health care workers to monitor the presence of AZT in low resource settings.
