Related Experiment Video
Updated: Nov 20, 2025
Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Published on: July 27, 2015
Similar performance fatigability and neuromuscular responses following sustained bilateral tasks above and below
Joshua L Keller1, Terry J Housh2, John Paul V Anders2
1Department of Health, Kinesiology and Sport, College of Education and Professional Studies, University of South Alabama, 171 Jaguar Dr. HKS 1008, Mobile, AL, 36688, USA. jkeller@southalabama.edu.
Purpose:
The present study examined the magnitude of performance fatigability as well as the associated limb- and intensity-specific neuromuscular patterns of responses during sustained, bilateral, isometric, leg extensions above and below critical force (CF).
Methods:
Twelve women completed three sustained leg extensions (1 below and 2 above CF) anchored to forces corresponding to RPE = 1, 5, and 8 (10-point scale). During each sustained leg extension, electromyographic (EMG) and mechanomyographic (MMG) amplitude (AMP) and mean power frequency (MPF) were assessed from each vastus lateralis in 5% of time-to-exhaustion (TTE) segments. Before and after each sustained leg extension, the subjects completed maximal voluntary isometric contractions (MVIC), and the percent decline was defined as performance fatigability. Polynomial regression was used to define the individual and composite neuromuscular and force values versus time relationships. Repeated-measures ANOVAs assessed differences in performance fatigability and TTE.
Results:
The grand mean for performance fatigability was 10.1 ± 7.6%. For TTE, the repeated-measures ANOVA indicated that there was a significant (p < 0.05) effect for Intensity, such that RPE = 1 > 5 > 8. There were similar neuromuscular patterns of response between limbs as well as above and below CF. EMG MPF, however, exhibited decreases only above CF.
Conclusions:
Performance fatigability was unvarying above and below CF as well as between limbs. In addition, there were similar fatigue-induced motor unit activation strategies above and below CF, but peripheral fatigue likely contributed to a greater extent above CF.
