Related Experiment Video

Updated: Nov 20, 2025

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

845

Low Molecular Pectin Inhibited the Lipid Accumulation by Upregulation of METTL7B

Xiaojin Yang1, Yinghua Yuan2, Desheng Xie1

  • 1Department of Infectious Diseases, Shanghai Fifth People's Hospital, Fudan University, No. 128 Ruili Road, Shanghai, 200240, China.

Applied Biochemistry and Biotechnology
|January 23, 2021
PubMed

Insights

Low-molecular-weight citrus pectin (LCP) reduces liver fat accumulation by increasing METTL7B, adipose triglyceride lipase (ATGL), and carnitine palmitoyltransferase-1 (CPT-1) levels. This suggests LCP may prevent nonalcoholic fatty liver disease progression.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nutritional Science

Background:

  • Nonalcoholic fatty liver (NAFL) progression to nonalcoholic steatohepatitis is linked to lipid accumulation.
  • Targeting lipid accumulation is crucial for preventing NAFL progression.

Purpose of the Study:

  • To investigate the effect of low-molecular-weight citrus pectin (LCP) on lipid accumulation in liver cells.
  • To elucidate the underlying molecular mechanisms of LCP's action.

Main Methods:

  • An in vitro model using oleic acid (OA)-induced lipid deposition in HepG2 cells.
  • Oil Red O staining to quantify lipid accumulation.
  • RT-qPCR and RNA sequencing to analyze gene expression, including ATGL, CPT-1, and METTL7B.

Main Results:

  • LCP significantly reduced OA-induced lipid accumulation in HepG2 cells.
  • LCP treatment increased the expression of ATGL and CPT-1, key enzymes in lipid metabolism.
  • RNA sequencing identified METTL7B as a significantly upregulated gene, which, upon overexpression, also inhibited lipid accumulation and enhanced ATGL and CPT-1 levels.

Conclusions:

  • LCP inhibits lipid accumulation in liver cells, potentially by upregulating METTL7B.
  • The mechanism involves enhancing enzymes involved in triacylglycerol catabolism and fatty acid oxidation.
  • LCP shows promise as a therapeutic agent for managing fat accumulation in NAFL.
Keywords:
ATGLCPT-1Lipid accumulationLow molecular pectinMETTL7B

More Related Videos

Experimental Screening Protocols, Immunocytochemistry and Microscopy-based Imaging Techniques for Penium margaritaceum09:26

Experimental Screening Protocols, Immunocytochemistry and Microscopy-based Imaging Techniques for Penium margaritaceum

Published on: March 28, 2025

464
Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia11:06

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Published on: April 7, 2023

2.5K

Related Concept Videos

Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
4.3K
Lipid Absorption01:24

Lipid Absorption

Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
2.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.8K
Lipid Digestion01:06

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
97.1K
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
535