Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide identification of SNPs in microRNA genes and the SNP effects on microRNA target binding and biogenesis.

Human mutation·2011
Same author

A case of intimal sarcoma of the pulmonary artery successfully treated with chemotherapy.

International journal of clinical oncology·2011
Same author

Exome sequencing identifies frequent mutation of ARID1A in molecular subtypes of gastric cancer.

Nature genetics·2011
Same author

Energetic salts based on dipicrylamine and its amino derivative.

Chemistry (Weinheim an der Bergstrasse, Germany)·2011
Same author

Biophysical properties of slow potassium channels in human embryonic stem cell derived cardiomyocytes implicate subunit stoichiometry.

The Journal of physiology·2011
Same author

Natural variation of folate content and composition in spinach (Spinacia oleracea) germplasm.

Journal of agricultural and food chemistry·2011

Related Experiment Video

Updated: Nov 20, 2025

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain
06:52

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain

Published on: January 26, 2024

2.5K

Light-field microscopy for fast volumetric brain imaging.

Zhenkun Zhang1, Lin Cong2, Lu Bai1

  • 1Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Journal of Neuroscience Methods
|January 23, 2021
PubMed
Summary
This summary is machine-generated.

Light-field microscopy enhances neural recording throughput and speed for in vivo imaging. This technology captures volumetric data, enabling detailed study of brain activity in dynamic biological processes.

Keywords:
Calcium imagingFluorescence microscopyLight-field microscopyVolumetric imaging

More Related Videos

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
04:35

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

16.6K
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
08:49

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Published on: August 1, 2022

4.0K

Related Experiment Videos

Last Updated: Nov 20, 2025

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain
06:52

Author Spotlight: Advancing 3D Cytoarchitecture Analysis - Rapid Volumetric Reconstruction of the Human Brain

Published on: January 26, 2024

2.5K
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
04:35

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

16.6K
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
08:49

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Published on: August 1, 2022

4.0K

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Imaging

Background:

  • Understanding neural circuit function requires large-scale in vivo recordings.
  • Conventional optical microscopy faces limitations in throughput and volumetric imaging speed.
  • Existing methods struggle to capture rapid neural dynamics across large brain areas.

Purpose of the Study:

  • To review the principles and practical implementation of light-field microscopy (LFM) for biological imaging.
  • To explore LFM's potential for high-throughput, high-speed, volumetric neural recordings.
  • To discuss strategies for optimizing LFM in challenging imaging conditions like thick tissues.

Main Methods:

  • Summarizing principles of light-field microscopy.
  • Discussing practical considerations for experimental setup and data acquisition.
  • Reviewing strategies for handling scattering and background noise in thick samples.

Main Results:

  • Light-field microscopy offers parallelized optical signal collection, enabling simultaneous recording from multiple depths.
  • Its instantaneous volumetric imaging capability is suitable for capturing fast neural dynamics in whole organisms.
  • LFM has been successfully applied to model organisms like C. elegans and zebrafish.

Conclusions:

  • Light-field microscopy presents a powerful tool for advancing in vivo neuroscience research.
  • It overcomes limitations of traditional methods, allowing for unprecedented insights into neural population dynamics.
  • Further development and application of LFM will accelerate discoveries in brain function.