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Updated: Nov 20, 2025

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Published on: April 17, 2017
Effects of temperature and relative humidity in D2O on solid-state hydrogen deuterium exchange mass spectrometry
Rishabh Tukra1, Sam Gardner2, Elizabeth M Topp3
1Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, West Lafayette, IN 47907, United States.
Abstract:
Lyophilized powders containing myoglobin and various excipients were subjected to ssHDX-MS at different temperatures and D2O vapor activity (RH). Deuterium incorporation was fitted to a bi-exponential association model for each formulation and the dependence of regression parameters on temperature and RH was evaluated. Data fitted best to a simplified model in which the slow exponential term was considered invariant with temperature and RH while the fast exponential term retained its temperature and RH dependence. This suggests that rapid rate processes such as water vapor sorption and initial deuterium labeling may be more dependent on temperature and RH than slower processes such as sequential exchange and transport in the solid matrix.
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