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Related Experiment Videos

[Cryopreservation of circulating stem cells in large quantities].

Y Takaue1, T Watanabe, Y Kawano

  • 1Dept. of Pediatrics, University Hospital of Tokushima.

Gan to Kagaku Ryoho. Cancer & Chemotherapy
|March 1, 1988
PubMed
Summary

Peripheral blood mononuclear cells were collected and cryopreserved from children with cancer. This method successfully enriches hematopoietic stem cells for potential future use in cancer therapy.

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Area of Science:

  • Hematology
  • Cell Biology
  • Oncology

Context:

  • Leukapheresis is used to collect peripheral blood mononuclear cells (PBMCs) from pediatric patients with malignant disorders.
  • Cryopreservation of hematopoietic stem cells (HSCs) is crucial for potential future therapeutic applications.
  • Efficient enrichment and storage of HSCs are necessary to optimize cell therapy protocols.

Purpose:

  • To develop and evaluate a method for large-scale collection, enrichment, and cryopreservation of HSCs from pediatric cancer patients.
  • To assess the recovery rates of key progenitor cells (CFU-GM and CFU-GEMM) after the freeze-thaw process.
  • To establish a reliable method for storing viable stem cells for potential use in rescue surgery following chemotherapy.

Summary:

  • Peripheral blood mononuclear cells were collected via leukapheresis from children with malignancies.

Related Experiment Videos

  • Percoll gradient fractionation was employed to enrich for hematopoietic stem cells and reduce sample volume.
  • Cells were cryopreserved using a programmed freezer and stored in liquid nitrogen, allowing for reduced DMSO infusion volumes and increased storage efficiency.
  • Recovery rates for granulocyte-macrophage colony-forming units (CFU-GM) were 89%, and for granulocyte, erythroid, macrophage, megakaryocyte colony-forming units (CFU-GEMM) were 106% after freeze-thaw.
  • The study concludes that circulating stem cells can be collected and cryopreserved in large quantities without loss of functional capacity.

    • Impact:

    • This cryopreservation technique enables the storage of large quantities of viable hematopoietic stem cells from pediatric cancer patients.
    • The method facilitates reduced dimethyl sulfoxide (DMSO) volumes during infusion, potentially minimizing toxicity.
    • The successful cryopreservation supports further research into the use of these stem cells for rescue therapy after high-dose chemotherapy in pediatric oncology.