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Updated: Nov 20, 2025
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
Published on: March 9, 2018
Copper nanocluster-based sensor for determination of vancomycin in exhaled breath condensate: A synchronous
Elaheh Rahimpour1, Maryam Khoubnasabjafari2, Mohammad Bagher Hosseini3
1Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran; Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran.
Abstract:
A synchronous fluorescence spectroscopy (SFS) nanoprobe is developed for the determination of vancomycin in exhaled breath condensate (EBC) samples. The synthesized nanoprobe is copper nanoclusters (Cu NCs) and its SFS peak is located at 405 nm with Δλ = 80. The affinity of Cu NCs to complex formation with vancomycin results in blocking non-radiative e-/h+ recombination defect sites on the surface of NCs and consequently enhancing the SFS signal intensity. Central composite design and response surface methodology is used for the optimization of reaction conditions. Under the optimized conditions, a linear relationship is found between the SFS intensity and the concentration of vancomycin in the range of 0.1-8 μg/mL. The validated method is applied for the determination of vancomycin in EBC of newborns receiving vancomycin treatment.
