Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.1K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.1K
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

3.6K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
3.6K
Protein Modifications in the RER01:26

Protein Modifications in the RER

6.3K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Eosinophil Essentials: Identification and Assessment of Activity.

Journal of leukocyte biology·2026
Same author

Eosinophil-derived COX-2 protects against experimental colitis through the PGE<sub>2</sub>-IL-22 axis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Tezepelumab Reduces Alarmin Cytokine Levels in Upper Airway Epithelial Cells in Patients With Severe Asthma.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026
Same author

Activation of donor-derived eosinophils during static storage contributes to primary graft dysfunction after lung transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

IL-33 blockade attenuates vascular inflammation in a mouse model of Kawasaki disease vasculitis.

Clinical and experimental immunology·2026
Same author

Eosinophil-derived COX-2 protects against experimental colitis through the PGE<sub>2</sub>-IL-22 axis.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 20, 2025

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis
03:23

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Published on: May 10, 2024

1.1K

Eosinophil Shape Change and Secretion.

Sergei I Ochkur1, Elizabeth A Jacobsen1, Paige Lacy2

  • 1Division of Allergy, Asthma and Clinical Immunology, Mayo Clinic Arizona, Scottsdale, AZ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 24, 2021
PubMed
Summary
This summary is machine-generated.

Analyzing eosinophil shape change and mediator secretion, specifically eosinophil peroxidase (EPX), offers insights into immune responses. This study details methods for assessing these changes after chemotactic stimulation in vitro and ex vivo.

Keywords:
ChemokineChemotaxisConfocal microscopyDegranulationEosinophil peroxidaseEotaxinPlatelet-activating factorSecretion

More Related Videos

Imaging FITC-dextran as a Reporter for Regulated Exocytosis
04:50

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Published on: June 20, 2018

13.0K
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
08:44

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

6.3K

Related Experiment Videos

Last Updated: Nov 20, 2025

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis
03:23

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Published on: May 10, 2024

1.1K
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
04:50

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Published on: June 20, 2018

13.0K
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
08:44

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

6.3K

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Eosinophils are key immune cells involved in allergic responses and host defense.
  • Eosinophil activation involves significant shape alterations and the release of granule contents, such as eosinophil peroxidase (EPX).
  • Understanding these responses is crucial for diagnosing and treating eosinophil-related inflammatory diseases.

Purpose of the Study:

  • To establish and validate methods for analyzing eosinophil shape change in response to chemotactic stimuli.
  • To present and compare two distinct assays for quantifying eosinophil peroxidase (EPX) secretion.
  • To provide a framework for assessing eosinophil activation from both in vitro and ex vivo samples.

Main Methods:

  • Confocal microscopy was employed to visualize and quantify eosinophil shape changes.
  • Degranulation was assessed using two methods: an enzyme assay with o-phenylenediamine (OPD) and an enzyme-linked immunosorbent assay (ELISA).
  • Experiments compared unstimulated eosinophils with those stimulated by chemotactic factors like platelet-activating factor (PAF) and CCL11 (eotaxin-1).

Main Results:

  • Eosinophils exhibited distinct morphological changes upon stimulation with chemotactic factors.
  • Both OPD and ELISA assays successfully measured EPX secretion, demonstrating their utility in quantifying degranulation.
  • The study successfully differentiated between stimulated and unstimulated eosinophil responses in terms of shape and mediator release.

Conclusions:

  • Confocal microscopy and specific degranulation assays provide robust methods for studying eosinophil activation.
  • These techniques are valuable for investigating eosinophil function in various immunological contexts.
  • The described approaches facilitate the assessment of eosinophil responses in both laboratory and clinical settings.