Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

1.5K
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
1.5K
Asthma-II: Pathophysiology and Classification01:26

Asthma-II: Pathophysiology and Classification

3.6K
Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
3.6K
Allergic Reactions02:06

Allergic Reactions

30.1K
Overview
30.1K
Antibody Structure01:10

Antibody Structure

63.0K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
63.0K
Allergic Drug Reactions01:27

Allergic Drug Reactions

1.2K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.2K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

974
Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
974

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The alpha-gal syndrome: Understanding the role of tick bites, and the delays in severe anaphylaxis.

Allergy and asthma proceedings·2026
Same author

Melittin in AllergoOncology: From Honey Bee Venom to Anti-Cancer Therapeutic Innovation-An EAACI Position Paper.

Allergy·2026
Same author

Meaningful outcomes in food allergy immunotherapy: Measuring what counts.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2026
Same author

Chronic rhinosinusitis with nasal polyps (CRSwNP) through an epigenetic lens: Plasmacytoma variant translocation 1 (PVT1) as a potential biomarker and therapeutic target.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2026
Same author

Mast Cells in Acute COVID-19 Patients.

Allergy·2026
Same author

Restoration of Keratinocyte Homeostasis Drives Resolution of Skin Inflammation.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 20, 2025

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

13.5K

Update on Eosinophil Interaction with Mast Cells: The Allergic Effector Unit.

Roopesh Singh Gangwar1, Hadas Pahima1, Pier Giorgio Puzzovio1

  • 1Pharmacology and Experimental Therapeutics Unit, Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.

Methods in Molecular Biology (Clifton, N.J.)
|January 24, 2021
PubMed
Summary

Mast cells and eosinophils form an "allergic effector unit" that drives chronic allergic inflammation through bidirectional communication. This interaction, crucial in late and chronic allergic reactions, can be studied in vitro.

Keywords:
Allergic effector unitBMEosBMMCsCo-cultureMast cell-eosinophil interactionsMurine AEU

More Related Videos

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation
08:25

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

Published on: April 27, 2021

2.5K
Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis
03:23

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Published on: May 10, 2024

1.1K

Related Experiment Videos

Last Updated: Nov 20, 2025

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

13.5K
An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation
08:25

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

Published on: April 27, 2021

2.5K
Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis
03:23

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Published on: May 10, 2024

1.1K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Allergic reactions involve mast cells and eosinophils as key effector cells.
  • Chronic allergic inflammation arises when allergic reactions fail to resolve, characterized by persistent mast cell and eosinophil presence.

Purpose of the Study:

  • To define and characterize the bidirectional interactions between mast cells and eosinophils in allergic inflammation.
  • To introduce the concept of the "allergic effector unit" to describe this cellular crosstalk.

Main Methods:

  • Investigated the interactions between mast cells (MCs) and eosinophils (Eos).
  • Utilized a customized in vitro co-culture system with human or mouse-derived MCs and Eos.
  • Examined cell-cell contacts via receptors (e.g., CD48, 2B4) and released mediators.

Main Results:

  • Mast cells and eosinophils exhibit bidirectional cross-talk in inflamed tissues during late and chronic allergic phases.
  • This interaction, termed the "allergic effector unit," is mediated by physical contact and secreted mediators.
  • The study established an in vitro model to study this unit.

Conclusions:

  • The "allergic effector unit" is a critical component in the pathogenesis of chronic allergic inflammation.
  • Understanding this unit's mechanisms is vital for developing targeted therapies for allergic diseases.
  • In vitro co-culture systems provide a valuable platform for studying MC-Eos interactions.