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Updated: Nov 20, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Multiplex MicroRNA Detection on a Surface-Functionalized Power-Free Microfluidic Chip.

Ryo Ishihara¹, Ryoichi Kitane², Yoshitsugu Akiyama³

¹Faculty of Medicine, Juntendo University, 1-1 Hirakagakuendai, Inzai, Chiba, 270-1695, Japan. r-ishihara@juntendo.ac.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|January 25, 2021

Summary

This summary is machine-generated.

A novel microfluidic chip enables multiplex microRNA detection for cancer diagnostics. This surface-functionalized, power-free device offers sensitive and rapid biomarker identification for point-of-care testing.

Keywords:

Surface-functionalized power-free microfluidic chip biomarkers cancer multiplex microRNA detection point-of-care testing

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Last Updated: Nov 20, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

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Published on: March 13, 2017

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Area of Science:

Biomarker Discovery
Microfluidics
Molecular Diagnostics

Background:

Circulating microRNAs (miRNAs) are valuable cancer biomarkers due to their association with cancer type and stage.
Existing multiplex miRNA detection methods require further optimization for clinical applications.

Purpose of the Study:

To develop a novel surface-functionalized, power-free microfluidic chip (SF-PF microchip) for sensitive and simultaneous multiplex microRNA detection.
To evaluate the performance of the SF-PF microchip for cancer biomarker analysis.

Main Methods:

Functionalization of microchannel inner surfaces using electron beam-induced graft polymerization and capture probe DNA immobilization.
Development of line-type and stripe-type SF-PF microchips for duplex and triplex miRNA detection.
Assessment of detection limits, sample volume, and detection time for miRNA analysis.

Main Results:

Simultaneous and specific duplex miRNA detection achieved with low detection limits (19.1 nmol L⁻¹ for hsa-miR-16, 47.6 nmol L⁻¹ for hsa-miR-500a-3p).
Simultaneous and specific triplex miRNA detection demonstrated on the stripe-type SF-PF microchip.
Minimal sample volume (0.5 μL) and rapid detection time (17 min) achieved.

Conclusions:

The developed SF-PF microchip enables sensitive, simultaneous, and rapid multiplex miRNA detection.
This technology holds promise for advancing cancer point-of-care testing (POCT) by detecting up to six miRNA types.
The SF-PF microchip offers a power-free, low-sample-volume solution for biomarker analysis.