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Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Bio-imaging with the helium-ion microscope: A review.

Matthias Schmidt1, James M Byrne2, Ilari J Maasilta3

  • 1Helmholtz-Centre for Environmental Research GmbH - UFZ, Permoserstraße 15, 04318 Leipzig, Germany.

Beilstein Journal of Nanotechnology
|January 25, 2021
PubMed
Summary
This summary is machine-generated.

Scanning helium-ion microscopy (HIM) offers sub-nanometre resolution for biological imaging. This technique provides superior surface sensitivity and depth of field, enabling detailed visualization of tiny biological structures.

Keywords:
HIMHIM-SIMSbio-imagingflood gunhelium-ion microscopyhigh resolutionionofluorescense

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Area of Science:

  • Biological Imaging
  • Microscopy Techniques
  • Materials Science

Background:

  • Scanning helium-ion microscopy (HIM) provides sub-nanometre resolution, surpassing conventional field-emission scanning electron microscopy (FE-SEM).
  • Helium ions offer advantages over electrons, including enhanced surface sensitivity, greater depth of field, and charge compensation for non-conductive biological samples.

Purpose of the Study:

  • To provide a comprehensive overview of published literature on HIM applications for biological specimens.
  • To discuss the technical features of HIM and highlight emerging advances in biological research.

Main Methods:

  • Review of scientific literature demonstrating HIM for imaging biological specimens.
  • Analysis of technical capabilities of HIM, including milling and coupling with other analytical techniques.

Main Results:

  • HIM has been successfully applied to various biological fields, including medicine, plants, microbiology, virology, and geomicrobiology.
  • The technique enables high-resolution imaging and milling of biological objects, such as viruses.
  • Integration of secondary ion mass spectrometry (SIMS) and ionoluminescence with HIM opens new research avenues.

Conclusions:

  • HIM is a powerful high-resolution imaging technique for diverse biological samples.
  • Its unique capabilities, including milling and advanced analytical integrations, offer significant potential for future biological research.