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Updated: Nov 20, 2025

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Published on: September 3, 2009
Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic
Ajit Kumar1,2, Mirza Ghazanfar Ullah Ghazi1, Bhim Singh1
1Department of Animal Ecology and Conservation Biology, Wildlife Institute of India, Dehradun, India.
Abstract:
We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning. These primers amplified 301-1599 bp DNA fragments in various combinations. The method described may assist in the sequencing of either complete gene from moderate to good quality DNA or shorter fragment from degraded DNA.

