Related Experiment Video
Updated: Nov 20, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Complete mitochondrial genome sequence of Emberiza sulphurata (Emberizidae: Emberiza)
Mu-Yeong Lee1, Hey Sook Jeon1, Junghwa An1
1Animal Resources Division, National Institute of Biological Resources, Incheon, Republic of Korea.
Abstract:
The Japanese yellow bunting (Emberiza sulphurata) is considered to be an endangered species. Here, the complete mitochondrial genome of E. sulphurata (16,797 bp in length) was determined. The genome consists of 37 genes (13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes) and one control region (D-loop), and so is highly similar in architecture to the typical vertebrate mtDNA genome. The base composition of the mtDNA was A (29.9%), C (32.6%), G (14.5%), and T (23.0%), so the percentage of A and T (52.9%) was slightly higher than that of G and C. All the genes in E. sulphurata were encoded on the H-strand, except for the genes for the ND6 subunit and eight tRNAs, which were encoded on the L-stand. Phylogenetic analysis using Emberizidae mitogenomes revealed that E. sulphurata was grouped into the family Emberizidae and that E. spodocephala is the most closely related species.
Related Concept Videos
Export of Mitochondrial and Chloroplast Genes
Animal Mitochondrial Genetics
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Modern Molecular Taxonomy
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...

