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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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The complete mitochondrial genome of the Basidiomycete fungus Pleurotus cornucopiae (Paulet) Rolland

Li-Ming Xu1, Damien Daniel Hinsinger2, Guo-Feng Jiang2,3

  • 1Biology Institute, Guangxi Academy of Sciences, Nanning, Guangxi, PR China.

Mitochondrial DNA. Part B, Resources
|January 25, 2021
PubMed

Insights

The complete mitochondrial genome of the edible fungus *Pleurotus cornucopiae* was sequenced and analyzed. This analysis provides insights into fungal taxonomy and evolution within the Agaricales order.

Area of Science:

  • Mycology
  • Genomics
  • Bioinformatics

Background:

  • Pleurotus cornucopiae is a commercially significant edible and medicinal fungus.
  • Understanding fungal mitochondrial genomes is crucial for evolutionary and taxonomic studies.

Purpose of the Study:

  • To determine and analyze the complete mitochondrial genome of *Pleurotus cornucopiae*.
  • To investigate the phylogenetic position of *P. cornucopiae* within the Agaricales order.

Main Methods:

  • Whole mitochondrial genome sequencing and assembly.
  • Comparative genomic analysis with related fungal species.
  • Phylogenetic analysis using complete mitochondrial genome data.

Main Results:

  • The mitogenome of *P. cornucopiae* is 72,134 bp with 26.7% GC content.
  • It contains 14 protein-coding genes, 2 rRNA genes, 1 ribosomal protein gene (rps3), and 24 tRNA genes.
  • Gene content and order are similar to *P. ostreatus* and *P. eryngii*.
  • Phylogenetic analysis places *P. cornucopiae* within Agaricales, closely related to *P. ostreatus* and *P. eryngii*.

Conclusions:

  • The mitochondrial genome sequence of *P. cornucopiae* is a valuable resource for fungal taxonomy and evolutionary research.
  • This study clarifies the evolutionary relationships within the Pleurotaceae and Agaricales.
Keywords:
FungiPleurotus cornucopiaeevolutionmitogenomemushroom

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