Related Experiment Video
Updated: Nov 20, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
The complete chloroplast genome sequence of Cremastra appendiculata (Orchidaceae) revealed by next-generation
Shao Li Mao1,2, Yafu Zhou1,2, Hao Yuan3
1Xi'an Botanical Garden of Shaanxi Province/Institute of Botany of Shaanxi Province, Xi'an, China.
Abstract:
The complete chloroplast genome sequence of Cremastra appendiculata, a rare and endangered species of China, was determined by Illumina pair-end sequencing. The results showed that the complete plastid genome was 160,494 bp in length, containing a large single copy (LSC) of 88,249 bp and a small single copy (SSC) of 21,457 bp, which were separated by a pair of 25,394 bp inverted repeats (IRs). A total of 132 unique genes were annotated, including 86 protein coding genes, 38 tRNA genes, and 8 rRNA genes. Among these genes, 18 genes contained one or two introns. The overall GC contents of the plastid genome were 36.9%, and in the IR regions, LSC and SSC were 43.8, 34.4 and 30.9%, respectively. Maximum likelihood analysis revealed that the new sequenced species C. appendiculata was closer to the species in Cattleya and formed one clade with a high bootstrap value at the middle of the phylogenetic tree of Orchidaceae.
More Related Videos
Related Concept Videos
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Anatomy of Chloroplasts
The Anatomy of Chloroplasts
Structure of...
Asexual Reproduction
Evolutionary Relationships through Genome Comparisons
Export of Mitochondrial and Chloroplast Genes

