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Updated: Nov 20, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

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Published on: January 14, 2021

Protocol for isolation of spermatids from mouse testes.

Chang Rok Kim¹, Taichi Noda², Yuki Okada³

¹Research Center for Epigenetic Code and Diseases, Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea.

|January 25, 2021

Summary

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Researchers developed a simple method using unit gravity sedimentation to isolate mouse spermatid fractions. This technique aids in studying protein and transcript changes during spermiogenesis, the process of sperm development.

Area of Science:

Reproductive Biology
Developmental Biology
Cell Biology

Background:

Spermiogenesis involves the transformation of post-meiotic spermatids into spermatozoa.
Understanding protein and transcript expression changes during spermiogenesis requires isolating distinct spermatid developmental stages.
Current methods for isolating these fractions can be complex.

Purpose of the Study:

To present a straightforward protocol for isolating spermatid fractions from mouse testes.
To facilitate the analysis of molecular changes during spermiogenesis.

Main Methods:

Unit gravity sedimentation in a Bovine Serum Albumin (BSA) density gradient was employed.
This method allows for the separation of spermatid fractions based on their developmental stage.

Keywords:

Cell Biology Cell isolation Model Organisms

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Last Updated: Nov 20, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

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Published on: January 14, 2021

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

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Published on: September 17, 2019

Mouse Round Spermatid Injection

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Published on: January 26, 2024

Main Results:

A simple and effective method for isolating spermatid fractions was successfully established.
The isolated fractions represent distinct stages of spermiogenesis.

Conclusions:

This protocol provides a valuable tool for researchers studying spermiogenesis.
It enables detailed analysis of transcript and protein expression dynamics during sperm development.