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Related Experiment Video

Updated: Nov 20, 2025

Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
18:11

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SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse.

Sydney J Reitz1, Andrew D Sauerbeck1, Terrance T Kummer1

  • 1Washington University School of Medicine, Department of Neurology, St. Louis, MO 63110, USA.

STAR Protocols
|January 25, 2021
PubMed
Summary

A new method called SEQUIN allows researchers to analyze individual synaptic components in the brain. This super-resolution imaging technique quantifies synaptic loci and their properties across large brain regions.

Keywords:
MicroscopyNeuroscience

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biophysics

Background:

  • Synapses are vital for brain function and are implicated in numerous neurological diseases.
  • Current methods for analyzing synaptic components in situ are limited in scope and accessibility.
  • There is a need for advanced techniques to study individual synaptic structures and their molecular makeup.

Purpose of the Study:

  • To introduce SEQUIN, a novel protocol for the comprehensive analysis of synaptic loci.
  • To enable quantification of molecular and nanostructural properties of individual synapses.
  • To overcome limitations of existing methods for in situ synaptic analysis.

Main Methods:

  • Utilizes a widely available super-resolution microscopy platform.
  • Combines advanced image processing and analysis techniques.
  • Applies the SEQUIN protocol for quantifying synaptic loci in large brain regions.

Main Results:

  • SEQUIN successfully quantifies synaptic loci across extensive brain areas.
  • The method characterizes molecular and nanostructural properties at both individual and population levels.
  • Provides a detailed protocol for synaptic loci quantification.

Conclusions:

  • SEQUIN offers a powerful and accessible solution for detailed synaptic analysis.
  • This protocol advances the study of brain function and disease at the synaptic level.
  • Enables high-throughput characterization of synaptic components in situ.