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Accumulation of Mitochondrial DNA Microsatellite Instability in Malaysian Patients with Primary Central Nervous
Siti Radzak1, Zulaikha Khair, Farizan Ahmad
1Sains Malaysia University, School of Medical Sciences, Health Campus, Department of Neurosciences, 16150 Kubang Kerian, Kelantan, Malaysia.
Aim:
To determine the mitochondrial microsatellite instability (mtMSI) status in a series of Malaysian patients with brain tumors. Furthermore, we analyzed whether the mtMSI status is associated with the clinicopathological features of the patients.
Material And Methods:
Forty fresh frozen tumor tissues along with blood samples of brain tumor patients were analyzed for mtMSI by PCR amplification of genomic DNAs, and the amplicons were directly sequenced in both directions using Sanger sequencing.
Results:
Microsatellite analysis revealed that 20% (8 out of 40) of the tumors were mtMSI positive with a total of 8 mtMSI changes. All mtMSI markers were detected in D310 and D16184 of the D-loop region. Additionally, no significant association was observed between mtMSI status and clinicopathological features.
Conclusion:
The variations, specifically the mtMSI, suggest that the mitochondrial DNA (mtDNA) can be targeted for genomic alteration in brain tumors. Therefore, the specific role of mtDNA alteration in brain tumor development and prognosis requires further investigation.
