Related Experiment Video
Updated: Nov 20, 2025
08:34
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
2.7K
What Is the Adequate PRP Dose for an Effective Treatment? An In Vitro Experimental Study on the Skin
Metin Görgü1, Ali Gökkaya1, Ertuğrul Karanfil1
1Department of Plastic, Reconstructive and Aesthetic Surgery, Abant Izzet Baysal University, Gölköy, Bolu, Turkey.
Aesthetic Surgery Journal
|January 25, 2021
Summary
Determining the optimal platelet-rich plasma (PRP) injection volume is crucial for effective treatment. Injecting 0.03 mL of PRP per unit area maximizes its spread within the dermis, regardless of needle size.
Area of Science:
- Regenerative Medicine
- Dermatology
- Wound Healing
Background:
- Clinical outcomes of platelet-rich plasma (PRP) therapies vary due to inconsistent preparation and administration protocols.
- Key variables influencing PRP efficacy include preparation methods, platelet concentration, and activation status.
Purpose of the Study:
- To investigate the impact of injected PRP volume on treatment outcomes.
- To determine the optimal PRP volume for effective intradermal tissue treatment.
Main Methods:
- PRP was injected into surplus skin tissues from 20 abdominoplasty and mammoplasty patients.
- Fluorescein-stained PRP was administered intradermally using varying needle gauges and volumes (0.01, 0.03, 0.05 mL).
- Dye spread was quantified in horizontal and vertical planes to assess dermal distribution.
Main Results:
- Needle gauge did not influence the width or depth of PRP spread in the dermis.
- Increasing PRP injection volume to 0.03 mL significantly enhanced dermal spread (width and thickness).
Conclusions:
- Standardization of PRP injection volume per unit tissue is essential for reproducible research and clinical application.
- A 0.03 mL volume appears optimal for maximizing PRP dermal spread.
