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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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A Paper-Based Colorimetric Aptasensor for the Detection of Gentamicin.

Saipriya Ramalingam1, Christopher M Collier1, Ashutosh Singh1

  • 1School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada.

Biosensors
|January 26, 2021
PubMed
Summary

A new microfluidic paper biosensor detects gentamicin residues in milk. This rapid, visual test ensures milk safety for consumers and farmers, addressing public health concerns from veterinary antibiotic use.

Keywords:
aptamercolorimetric biosensorgentamicingold nanoparticlespaper microfluidics

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Area of Science:

  • Veterinary Science
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Antibiotic residues in milk pose public health risks and economic challenges.
  • Current detection methods are often not suitable for on-farm application.

Purpose of the Study:

  • To develop a rapid, on-farm detection system for gentamicin residues in milk.
  • To create a visual and reliable biosensor for ensuring milk safety.

Main Methods:

  • Design and development of a microfluidic paper biosensor.
  • Utilizing gold nanoparticles and localized surface plasmon resonance (LSPR) for visual detection.
  • Employing DNA aptamers for specific gentamicin binding.
  • Image J RGB analysis for result corroboration.

Main Results:

  • Proof-of-concept detection system for gentamicin in milk.
  • Visual feedback provided by LSPR properties of gold nanoparticles.
  • Camera-based limit of detection (LOD) of 300 nM for gentamicin.
  • Rapid assay with a reaction time of 2 minutes.

Conclusions:

  • The developed microfluidic paper biosensor offers a quick and reliable method for on-farm gentamicin detection in milk.
  • This technology can help ensure milk safety and compliance with regulatory limits.
  • The biosensor provides a visual readout, making it accessible for on-site use.