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Protein Loading into Spongelike PLGA Microspheres.

Yuyoung Kim1,2, Hongkee Sah1

  • 1College of Pharmacy, Ewha Womans University, 52 Ewhayeodaegil, Seodaemun-gu, Seoul 03760, Korea.

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|January 26, 2021
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Summary
This summary is machine-generated.

This study optimized self-healing microencapsulation for protein loading using poly-d,l-lactide-co-glycolide (PLGA) microspheres. Key parameters like pore characteristics and microsphere hydration significantly enhance protein encapsulation efficiency.

Keywords:
closed-poremicroencapsulationopen-porepoly-d,l-lactide-co-glycolideporous microspheresprotein

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Self-healing microencapsulation offers potential for improved protein delivery.
  • Poly-d,l-lactide-co-glycolide (PLGA) microspheres are a promising platform for this technology.
  • Optimizing protein loading into these microspheres is crucial for therapeutic applications.

Purpose of the Study:

  • To identify and evaluate critical parameters influencing protein loading efficiency in self-healing PLGA microspheres.
  • To investigate the impact of microsphere pore characteristics and hydration state on lysozyme loading.
  • To understand how process parameters affect the microstructure and morphology of the final microspheres.

Main Methods:

  • Utilized lysozyme as a model protein for loading studies.
  • Investigated the effect of preformed microsphere pore characteristics (open-pore, closed-pore, porosity).
  • Assessed the influence of different surfactants (e.g., cetyltrimethylammonium bromide, Tween 80) on loading efficiency.
  • Compared protein loading using dried versus wet preformed microspheres.

Main Results:

  • Microsphere pore characteristics and porosity significantly impacted lysozyme loading efficiency.
  • Surfactant type played a crucial role; cetyltrimethylammonium bromide enhanced loading more than Tween 80.
  • Wet microspheres demonstrated superior lysozyme loading capacity and reduced hydration time compared to dried microspheres.
  • The degree of microsphere hydration influenced the microstructure and morphology post self-healing.

Conclusions:

  • Critical parameters for self-healing microencapsulation of proteins into PLGA microspheres have been identified.
  • Optimizing microsphere hydration and pore structure is key to maximizing protein loading.
  • These findings provide a foundation for bespoke protein encapsulation strategies using self-healing PLGA microspheres.