Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

8.9K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.9K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

7.8K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Proteomes</i> Annual Report Card 2025.

Proteomes·2026
Same author

Monocyte Migration Emerges from a Divergent Chemokine Signaling Network.

bioRxiv : the preprint server for biology·2026
Same author

Reactive Ions in Aqueous Electrospray Microdroplet Experiments Originate from Electrical Discharge.

Journal of the American Chemical Society·2026
Same author

Investigation into the metabolomic effects of the non-canonical amino acid azetidine-2-carboxylic acid in neuroblastoma cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2026
Same author

The Emergence of Chirality in Time and Space: Transient Asymmetry in Supramolecular Polymers Triggered by Visible Light.

Journal of the American Chemical Society·2026
Same author

Analysis of amino acids in Mucuna pruriens supplements using hydrophilic interaction liquid chromatography-tandem mass spectrometry.

Amino acids·2026

Related Experiment Video

Updated: Nov 20, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.4K

Misincorporation Proteomics Technologies: A Review.

Joel R Steele1,2, Carly J Italiano2, Connor R Phillips2

  • 1Proteomics Core Facility and School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia.

Proteomes
|January 26, 2021
PubMed
Summary
This summary is machine-generated.

Detecting noncanonical amino acids in proteins is crucial for understanding diseases like proteinopathies. This review explores current technologies for Misincorporation Proteomics (MiP), highlighting the need for further advancements in this field.

Keywords:
misincorporationnon protein amino acidspost translational modifications

More Related Videos

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.7K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

963

Related Experiment Videos

Last Updated: Nov 20, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.4K
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.7K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

963

Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Disease Mechanisms

Background:

  • Proteinopathies arise from altered proteoform structures.
  • A key hypothesis suggests noncanonical amino acid misincorporation causes these detrimental structures.
  • Proteomic evidence for this hypothesis, specifically detecting noncanonical amino acids in peptides, is currently lacking.

Purpose of the Study:

  • To review existing technologies for investigating protein mistranslations and misincorporations.
  • To establish the framework for Misincorporation Proteomics (MiP).
  • To identify technological gaps and areas for future development in MiP.

Main Methods:

  • Review of current mass spectrometry techniques.
  • Assessment of sample enrichment and preparation strategies.
  • Evaluation of data analysis approaches and hyphenated techniques.

Main Results:

  • Current technologies, including mass spectrometry and advanced data analysis, show potential for detecting misincorporated amino acids.
  • The integration of various approaches (hyphenation) is a promising strategy.
  • Significant limitations and a need for refinement exist across all explored technological domains.

Conclusions:

  • Despite technological advancements, direct proteomic evidence for noncanonical amino acid misincorporation remains challenging to obtain.
  • Further development and optimization of mass spectrometry, sample handling, and data interpretation are essential for advancing Misincorporation Proteomics.
  • Establishing robust MiP methodologies is critical for validating hypotheses about proteinopathies and related diseases.