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Updated: Nov 20, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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Visible Light Photochemical Reactions for Nucleic Acid-Based Technologies.

Bonwoo Koo1, Haneul Yoo1, Ho Jeong Choi1

  • 1Department of Chemistry, Chungbuk National University, Cheongju 28644, Korea.

Molecules (Basel, Switzerland)
|January 26, 2021
PubMed
Summary

Visible light photochemistry enables novel nucleic acid manipulations for medicinal chemistry and chemical biology. This review covers recent advances in DNA photocleaving, photoligation, sensors, and functional molecule release.

Keywords:
nucleic acid-based technologiesnucleic acidsphotochemical reactionstemplated reactionvisible light

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Area of Science:

  • Chemical Biology
  • Medicinal Chemistry
  • Organic Chemistry

Background:

  • Nucleic acid modifications are crucial for developing new technologies in medicine and biology.
  • Visible light photochemistry offers mild and efficient methods for manipulating nucleic acids.

Purpose of the Study:

  • To review recent developments in visible light photochemical reactions involving nucleic acids since 2010.
  • To highlight applications of these reactions in designing nucleic acid-based technologies.

Main Methods:

  • Literature review of visible light photochemical reactions applied to nucleic acids.
  • Focus on advancements from 2010 onwards.
  • Categorization of applications including DNA cleavage, ligation, sensing, and library synthesis.

Main Results:

  • Visible light-driven reactions facilitate diverse nucleic acid manipulations.
  • Applications include DNA photocleaving, DNA photoligation, and nucleic acid sensor development.
  • Emerging uses in functional molecule release and DNA-encoded libraries.

Conclusions:

  • Visible light photochemistry is a powerful tool for nucleic acid manipulation.
  • Recent advances have expanded its utility in creating innovative nucleic acid-based technologies.
  • This field holds significant promise for future applications in chemistry and biology.