Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electroacupuncture regulates detrusor connexin 43 via protein kinase A pathway in a rat model of neurogenic bladder after spinal cord injury.

The journal of spinal cord medicine·2026
Same author

Direct Cardiac T1 Mapping with Subspace Modeling and Free-breathing Data Acquisition.

IEEE transactions on bio-medical engineering·2026
Same author

The ATP-P2X signaling pathway mediates the effect of electroacupuncture on excessive bladder detrusor muscle contraction in a rat model of neurogenic bladder.

Acupuncture in medicine : journal of the British Medical Acupuncture Society·2026
Same author

Chemical proteomics reveals sinomenine's anti-inflammatory mechanism through serum protein covalent modification.

Chinese medicine·2026
Same author

ABIGX: A Unified Framework for eXplainable Fault Detection and Classification.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Selective regulation of partial nitrification/denitrification via light irradiation: Microbial niche differentiation and metabolic adaptation mechanisms.

Water research·2026

Related Experiment Video

Updated: Nov 20, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.1K

Laser particles with omnidirectional emission for cell tracking.

Shui-Jing Tang1,2, Paul H Dannenberg1,3, Andreas C Liapis1

  • 1Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.

Light, Science & Applications
|January 26, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed omnidirectional microlaser particles by adding light scatterers. This innovation enables continuous, high-signal tracking of individual cells within living tissues for extended periods, overcoming previous limitations.

More Related Videos

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.5K
Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction
10:03

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

Published on: October 25, 2012

11.8K

Related Experiment Videos

Last Updated: Nov 20, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.1K
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.5K
Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction
10:03

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

Published on: October 25, 2012

11.8K

Area of Science:

  • Biophotonics
  • Cellular Imaging
  • Materials Science

Background:

  • Tracking individual cells is vital for understanding complex biological systems and heterogeneous cell populations.
  • Microlaser particles offer potential for high-throughput single-cell analysis but suffer from direction-dependent emission.
  • Random particle orientation within cells leads to signal fluctuations and tracking failures in conventional microlasers.

Purpose of the Study:

  • To develop a general solution for overcoming the direction-dependent emission of microlaser particles.
  • To create omnidirectional microlaser probes for robust, continuous single-cell tracking in dynamic biological environments.
  • To enhance the signal-to-noise ratio and reliability of microlaser-based cell tracking.

Main Methods:

  • Incorporation of nanoscale light scatterers into microdisk lasers.
  • Two schemes were developed: introducing boundary defects or a scattering layer into microdisk lasers.
  • Testing of the modified microlasers' emission patterns and performance in live cells in vitro.

Main Results:

  • The modified microlasers exhibited omnidirectional emission, significantly improving the minimum-to-maximum intensity ratio from 0.007 to over 0.23.
  • Omnidirectional laser particles were successfully tracked continuously in live cells for up to 2 hours with high signal-to-noise ratios.
  • Conventional microlasers showed frequent signal loss, leading to tracking failures under similar conditions.

Conclusions:

  • Incorporating nanoscale light scatterers provides a general and effective method for achieving omnidirectional emission in microlasers.
  • Omnidirectional microlaser particles represent a significant advancement for reliable, long-term single-cell tracking in biological research.
  • This technology enhances the capability to analyze cellular dynamics in heterogeneous populations within living systems.