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Non-Proteasomal Urine Activity in Bladder Cancer
Natalia Gruba1, Lech Stachurski2, Adam Lesner1
1University of Gdansk, Faculty of Chemistry, Wita Stwosza 63 Street, PL, 80-308, Gdańsk, Poland.
Chemistry & Biodiversity
|January 26, 2021
Summary
Researchers developed a new urine test to detect bladder cancer (BC) by identifying specific enzyme activity. This non-invasive method shows promise for early bladder cancer detection, potentially reducing the need for invasive procedures.
Area of Science:
- Biochemistry
- Oncology
- Urology
Background:
- Bladder cancer (BC) is a prevalent malignancy with high recurrence rates and poor prognosis.
- Current diagnostic methods for BC can be invasive, expensive, and require regular re-examinations.
- BC often presents asymptomatically in its early stages, delaying diagnosis.
Purpose of the Study:
- To qualitatively determine specific urine enzyme activity in healthy individuals and bladder cancer patients.
- To explore the potential of using chromogenic substrates for BC detection.
- To establish a non-invasive method for identifying bladder cancer.
Main Methods:
- Utilized a specific peptide substrate (ABZ-Met-Lys-Val-Trp-ANB-NH2) for enzyme activity detection.
- Measured changes in absorbance at 410 nm to quantify substrate hydrolysis.
- Analyzed urine samples from both bladder cancer patients and healthy volunteers.
Main Results:
- Proteolytic enzyme activity was detected in the majority of urine samples from bladder cancer patients.
- No significant proteolytic activity was observed in urine samples from healthy volunteers.
- The assay demonstrated a clear distinction between the two groups based on enzyme activity.
Conclusions:
- The developed assay shows potential as a non-invasive diagnostic tool for bladder cancer.
- Urine enzyme activity determination using chromogenic substrates offers a promising approach for BC screening.
- Further research is warranted to validate this method for clinical application in bladder cancer diagnosis.
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