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Updated: Nov 20, 2025

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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Published on: March 31, 2021
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Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
Emerging Infectious Diseases
|January 26, 2021
Abstract:
We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.

