Related Experiment Video
Updated: Aug 9, 2026
08:38
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
Published on: October 30, 2017
Direct immunomagnetic quantification of lymphocyte subsets in blood
J E Brinchmann1, F Vartdal, G Gaudernack
1Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Clinical and Experimental Immunology
|January 1, 1988
Summary
This study introduces a rapid method using magnetic microspheres and specific antibodies for precise lymphocyte subset counting in blood. This technique offers accurate quantification of immune cells for clinical applications.
Area of Science:
- Immunology
- Biotechnology
- Cell Biology
Background:
- Accurate quantification of lymphocyte subsets is crucial for diagnosing and monitoring various immune-related conditions.
- Existing methods for lymphocyte subset enumeration can be time-consuming and complex.
Purpose of the Study:
- To develop and validate a direct, fast, and accurate method for quantifying absolute numbers of lymphocyte subsets in blood.
- To utilize superparamagnetic polymer microspheres coated with monoclonal antibodies for cell isolation and counting.
Main Methods:
- Incubation of blood samples with subset-specific monoclonal antibody (MoAb)-coated microspheres.
- Isolation and washing of microsphere-rosetted cells using a magnetic field.
- Detachment of microspheres, staining of cell nuclei with acridine orange, and counting via immunofluorescence microscopy.
Main Results:
- Reproducible absolute counts for CD2, CD4, CD8, and CD19 lymphocyte subsets were achieved.
- The results showed a close correlation with a validated indirect quantification method.
- The method demonstrated efficiency in direct and rapid quantification.
Conclusions:
- Superparamagnetic microsphere-based immunofluorescence offers a reliable and efficient approach for absolute lymphocyte subset quantification.
- This method provides a valuable tool for immunological diagnostics and research.
- The technique streamlines cell counting, improving speed and accuracy in clinical settings.
