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Published on: January 5, 2017
Sesamin protects against DSS-induced colitis in mice by inhibiting NF-κB and MAPK signaling pathways
Shuang Chen1, Chun-Lei Zhang1, Hai-Qing Shen1
1College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China. 1054702402@qq.com 755146950@qq.com shenhq@jlu.edu.cn 58506011@qq.com 1257022851@qq.com 1347263464@qq.com 997190849@qq.com fubendong96@163.com yipengfei1982@126.com.
Objective:
To investigate the protective effects and mechanisms of sesamin (SES) on dextran sulfate sodium (DSS)-induced experimental colitis in mice.
Methods:
SES (50, 100, and 200 mg kg-1) were orally administered to C57BL/6 male mice after DSS instillation. The anti-inflammatory effect of SES on colonic damage was assessed by clinical, macroscopic, microscopic, and inflammatory signaling pathways.
Results And Conclusions:
It could be found that bodyweight and colon length of mice treated with DSS was significantly decreased while that were increased by SES treatment. SES treatment reduced the DAI values and improved the histopathology of the colon in the DSS-treated mice. SES also reduced TNF-α, IL-1β and IL-6 production caused by DSS. We also measured the expression of the phosphorylation of p65, IκB, p38, ERK and JNK protein and found that SES can alleviate colon damage via the NF-κB and MAPK signaling pathways. The findings of this study suggested that SES had anti-inflammatory effects on intestinal inflammation and can be used as a new therapeutic candidate for inflammatory bowel disease.
