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Published on: July 14, 2015
Enrichment of marine manganese-oxidizing microorganisms using polycaprolactone as a solid organic substrate
Masataka Aoki1, Yukina Miyashita2, P Thao Tran3
1Department of Civil Engineering, National Institute of Technology, Wakayama College, 77 Noshima, Nada, Gobo, Wakayama, 644-0023, Japan. aoki@wakayama-nct.ac.jp.
Objective:
Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolactone (PCL), an aliphatic polyester, as an effective solid organic substrate for the enrichment of marine Mn-oxidizing microorganisms.
Results:
We successfully obtained marine microbial enrichment with the capacity for dissolved Mn removal and MnOx production using PCL as a solid organic substrate. The removal of dissolved Mn by the Mn-oxidizing enrichment culture followed first-order kinetics with a rate constant of 0.014 h-1. 16S rRNA gene amplicon sequencing analysis revealed that the Mn-oxidizing enrichment culture was highly dominated by operational taxonomic units related to the bacterial phyla Cyanobacteria, Planctomycetes, and Proteobacteria.
Conclusions:
Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions.
