Role of potassium transporter KUP8 in plant responses to heavy metals

Heavy metal concentrations, which have been increasing over the last 200 years, affect soil quality and crop yields. These elements are difficult to eliminate from soils and may constitute a human health hazard by entering the food chain. Recently, we obtained a selection of mutants with different degrees of tolerance to a mixture of heavy metals (HMmix) in order to gain a deeper insight into the underlying mechanism regulating plant responses to these elements. In this study, we characterized the mutant obtained Atkup8 (in this work, Atkup8-2), which showed one of the most resistant phenotypes, as determined by seedling root length. Atkup8-2 is affected in the potassium transporter KUP8, a member of the high-affinity K⁺ uptake family KUP/HAK/KT. Atkup8-2 mutants, which are less affected as measured by seedling root length under HMmix conditions, showed a resistant phenotype with respect to WT seedlings which, despite their delayed growth, are able to develop true leaves at levels similar to those under control conditions. Adult Atkup8-2 plants had a higher fresh weight than WT plants, a resistant phenotype under HMmix stress conditions and lower levels of oxidative damage. KUP8 did not appear to be involved in heavy metal or macro- and micro-nutrient uptake and translocation from roots to leaves, as total concentrations of these elements were similar in both Atkup8-2 and WT plants. However, alterations in cellular K⁺ homeostasis in this mutant cannot be ruled out.