Pharmacokinetics of Nitrate and Nitrite Following Beetroot Juice Drink Consumption

Nitrate (NO₃ ^-)-rich beetroot (BR) juice supplementation has been shown to improve cardiovascular function via reduction to nitrite (NO₂ ^-) and then to the bioactive molecule nitric oxide (NO). However, limited research exists for the role of inorganic NO₂ ^- that is contained naturally within BR.

As BR juice can naturally contain both NO₃ ^- and NO₂ ^- the objective of this study was to evaluate the individual effects of NO₃ ^- and NO₂ ^- consumed from BR on plasma [NO₃ ^-]/[NO₂ ^-] and their subsequent effects on various cardiovascular measures.

In four separate treatments, 11 healthy adults consumed 250 mL of BR containing one of the following: (i) high NO₃ ^-, low NO₂ ^- (HL; 572 mg NO₃ ^-, 32 mg NO₂ ^-); (ii) medium NO₃ ^-, medium NO₂ ^- (MM; 280 mg NO₃ ^-, 237 mg NO₂ ^-); (iii) low NO₃ ^-, medium NO₂ ^- (LM; 43 mg NO₃ ^-, 262 mg NO₂ ^-); (iv) placebo (PL; low NO₃ ^-, low NO₂ ^-: 8 mg NO₃ ^-, 5.8 mg NO₂ ^-). Plasma [NO₃ ^-]/[NO₂ ^-], blood pressure, heart rate, mean arterial pressure (MAP), cardiac output and stroke volume were measured at baseline and every hour or second hour for 6 h post-BR consumption.

Ingestion of the HL and MM BR increased plasma [NO₂ ^-] and [NO₃ ^-] after 2 h, with both remaining elevated after 6 h (p < 0.05). LM increased plasma [NO₃ ^-] (p < 0.05) but did not increase plasma [NO₂ ^-] compared to PL (p = 0.177). MAP was lower following the consumption of HL at 4 h and LM at 6 h (p < 0.05).

Inorganic NO₃ ^- consumption is the critical factor in elevating plasma [NO₃ ^-] and [NO₂ ^-]; however, both NO₂ ^- and NO₃ ^- show potential to reduce MAP. The known reduction of systolic blood pressure (SBP)/diastolic blood pressure (DBP) following NO₃ ^- supplementation was not observed, making it unclear if NO₂ ^- contributes to a reduction in SBP/DBP alongside NO₃ ^-.