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Related Experiment Videos

Free sphingoid bases in normal murine tissues.

T Kobayashi1, K Mitsuo, I Goto

  • 1Department of Neurology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

European Journal of Biochemistry
|March 15, 1988
PubMed
Summary

Free sphingoid bases were detected in mouse tissues, with lungs having the highest concentrations. These compounds may play roles in sphingolipid metabolism and cellular regulation.

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Area of Science:

  • Biochemistry
  • Lipid Metabolism
  • Cellular Biology

Background:

  • Sphingoid bases are crucial components of cellular membranes.
  • Previously, free sphingoid bases were not considered to occur naturally in tissues.

Purpose of the Study:

  • To detect and quantify free sphingoid bases in murine tissues.
  • To identify the molecular species and distribution of free sphingoid bases.
  • To elucidate the potential metabolic roles of free sphingoid bases.

Main Methods:

  • Detection using high-performance liquid chromatography (HPLC) after derivatization with o-phthalaldehyde.
  • Purification using lipid extraction, ion-exchange, and silicic acid chromatography.
  • Identification using thin-layer chromatography (TLC), HPLC, and fast-atom-bombardment mass spectrometry (FAB-MS).

Main Results:

  • Free sphingoid bases were detected in murine tissues at concentrations of 10-30 pmol/mg.
  • The lung exhibited the highest concentrations of sphingoid bases.
  • C18-sphingenine and C18-sphinganine were the most abundant molecular species, with variations in distribution across different tissues (e.g., C20-sphingenine in nervous tissues, 4-hydroxysphinganine in the kidney).
  • Galactosylsphingenine was identified in the spinal cord and sciatic nerve.

Conclusions:

  • Free sphingoid bases are present in normal murine tissues.
  • Their presence suggests a role as metabolic intermediates in sphingolipid synthesis or degradation.
  • These bases may be involved in regulating cellular metabolism.

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