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Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

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Related Experiment Video

Updated: Nov 19, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Multi-Q 2 software facilitates isobaric labeling quantitation analysis with improved accuracy and coverage.

Ching-Tai Chen¹, Jen-Hung Wang^2,3,4, Cheng-Wei Cheng⁵

¹Institute of Information Science, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115, Taiwan. ctchen@iis.sinica.edu.tw.

Scientific Reports

|January 27, 2021

Summary

This summary is machine-generated.

Multi-Q 2 enhances proteomic quantitation accuracy and coverage using isobaric labeling. This tool offers flexible algorithmic combinations for improved data analysis and biomarker discovery in mass spectrometry-based proteomics.

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Last Updated: Nov 19, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

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Published on: April 19, 2019

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

Area of Science:

Proteomics
Mass Spectrometry
Bioinformatics

Background:

Isobaric labeling in mass spectrometry-based proteomics enables multiplex quantitation.
Existing tools face limitations in quantitation coverage and accuracy.

Purpose of the Study:

Introduce Multi-Q 2, a novel software tool for isobaric-labeling quantitation.
Improve quantitation coverage and accuracy compared to state-of-the-art methods.

Main Methods:

Developed Multi-Q 2 with support for multiple proteomic data analysis platforms.
Integrated various quantitation algorithms, including ratio compression correction.
Enabled up to 336 algorithmic combinations for customized analysis.

Main Results:

Achieved the largest quantitation coverage and improved accuracy.
Demonstrated up to 12% improvement in quantitation coverage compared to MaxQuant and PatternLab.
Showcased enhanced sensitivity for biomarker discovery through complementary algorithmic combinations.

Conclusions:

Multi-Q 2 offers superior quantitation coverage and accuracy in proteomic studies.
Its flexible algorithmic combinations improve data analysis and biomarker identification.
The tool provides interactive visualization and clustering capabilities for enhanced data interpretation.