Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Light and temperature affect retinyl ester hydrolase activity and visual pigment composition.

A T Tsin1, J P Chambers

  • 1Division of Life Sciences, University of Texas, San Antonio 78285.

Experientia
|January 15, 1988
PubMed
Summary

Dim light and high water temperature increase retinyl ester hydrolase activity in goldfish retinas. This may enhance rhodopsin formation by increasing retinal chromophore availability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of common sedation agents on feline splenic size determined via ultrasonography.

New Zealand veterinary journal·2023
Same author

Indicators of dehydration in healthy 4- to 5-day-old dairy calves deprived of feed and water for 24 hours.

Journal of dairy science·2020
Same author

Effect of insulation with bubble wrap and an absorbent pad on heat loss in anaesthetised cats.

New Zealand veterinary journal·2020
Same author

Development of a veterinary antimicrobial stewardship online training program for Australian veterinarians: a national collaborative effort.

Australian veterinary journal·2019
Same author

Analgesic efficacy of articaine hydrochloride for velvet antler removal in red deer (<i>Cervus elaphus</i>) and analysis of drug residues in the harvested velvet antlers.

New Zealand veterinary journal·2019
Same author

Characterization of novel Mycobacterium tuberculosis pncA gene mutations in clinical isolates from the Ukraine.

Diagnostic microbiology and infectious disease·2018

Area of Science:

  • Biochemistry
  • Retinal Physiology
  • Aquatic Animal Adaptations

Background:

  • Retinyl ester hydrolase (REH) plays a key role in vitamin A metabolism within the retina.
  • Rhodopsin, the visual pigment, requires retinal chromophores derived from vitamin A.
  • Environmental factors like light and temperature can influence retinal biochemical processes.

Purpose of the Study:

  • To investigate the effect of combined dim light and high water temperature on REH activity in goldfish retina.
  • To explore the potential link between altered REH activity and rhodopsin synthesis under specific environmental conditions.

Main Methods:

  • Goldfish were exposed to controlled conditions of dim light and elevated water temperature.
  • Retinyl ester hydrolase activity in retinal homogenates was measured.

Related Experiment Videos

  • Enzyme activity was quantified using spectrophotometric assays.

    • Main Results:

    • A significant increase in retinyl ester hydrolase activity was observed in goldfish retinas under dim light and high water temperature.
    • This suggests an enhanced capacity for releasing retinol from retinyl esters.

    Conclusions:

    • The combination of dim light and high water temperature stimulates REH activity in goldfish retina.
    • This enzymatic response likely supports increased rhodopsin regeneration by providing more retinal chromophores, aiding vision in challenging environments.