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[Comparison of urine proteome in rats intraperitoneally infected by different microorganisms]
Wenshu Meng1, Yameng Zhang1, Yuanrui Hua1
1Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China.
Abstract:
Different microorganisms can cause intraperitoneal infection. This study was to distinguish different microbial infections by urine analysis. Rats were intraperitoneally injected with Escherichia coli, Staphylococcus aureus, and Candida albicans, separately. Urine samples were collected from rats at 0, 12, 36 and 72 h after infection. Urinary proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with the control (without infection), a total of 69 differential proteins were identified in rats injected with E. coli. A total of 31 differences proteins were identified in rats injected with S. aureus. A total of 38 differential proteins were identified in rats injected with C. albicans. Urine proteome was different when rats were infected by different microorganisms, suggesting that urine may have the potential for differential diagnosis of different intraperitoneal infections.
Insights
Urine analysis can distinguish between different microbial intraperitoneal infections. Profiling urinary proteins using liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed distinct protein changes for Escherichia coli, Staphylococcus aureus, and Candida albicans infections.
Area of Science:
- Microbiology
- Proteomics
- Medical Diagnostics
Background:
- Intraperitoneal infections can be caused by various microorganisms.
- Accurate and timely diagnosis is crucial for effective treatment.
- Distinguishing between different causative agents is a clinical challenge.
Purpose of the Study:
- To investigate the potential of urine analysis for differentiating microbial intraperitoneal infections.
- To identify unique urinary protein profiles associated with specific pathogens.
Main Methods:
- Rats were infected intraperitoneally with Escherichia coli, Staphylococcus aureus, or Candida albicans.
- Urine samples were collected at multiple time points post-infection (0, 12, 36, 72 hours).
- Urinary proteins were analyzed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).
Main Results:
- A total of 69 differential proteins were identified in rats infected with E. coli compared to controls.
- 31 differential proteins were identified in rats infected with S. aureus.
- 38 differential proteins were identified in rats infected with C. albicans.
- Distinct urinary proteome profiles were observed for each type of microbial infection.
Conclusions:
- The urine proteome differs significantly based on the causative microorganism of intraperitoneal infection.
- Urinary protein profiling shows promise as a non-invasive method for the differential diagnosis of intraperitoneal infections.
