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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Nov 19, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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CRISPR Systems for COVID-19 Diagnosis.

Hossein Rahimi1,2, Marziyeh Salehiabar3,4, Murat Barsbay5

  • 1Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

ACS Sensors
|January 27, 2021
PubMed
Summary

Clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics offer rapid, precise, and portable detection of COVID-19. These systems overcome limitations of traditional RT-qPCR, enabling faster intervention and disease control.

Keywords:
COVID-19CRISPRRT-qPCRSARS-CoV-2diagnosis

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Area of Science:

  • Molecular Diagnostics
  • Viral Detection
  • Biotechnology

Background:

  • The COVID-19 pandemic necessitates rapid and accurate diagnostic tools.
  • Traditional RT-qPCR methods face challenges including reagent scarcity, specialized equipment needs, and slow detection times.
  • Limitations in current diagnostics hinder timely intervention and disease management.

Purpose of the Study:

  • To review contemporary studies on CRISPR-based diagnostic systems for COVID-19 detection.
  • To highlight the advantages of CRISPR technology in molecular diagnostics.
  • To assess the potential of CRISPR systems in addressing the limitations of current COVID-19 testing.

Main Methods:

  • Review of recent scientific literature on CRISPR-based diagnostic platforms for SARS-CoV-2.
  • Analysis of studies focusing on the performance characteristics of CRISPR-based COVID-19 detection.
  • Evaluation of the technical requirements and operational benefits of CRISPR diagnostic systems.

Main Results:

  • CRISPR-based diagnostics demonstrate high speed, achieving results within 30 minutes from raw samples.
  • These systems offer high sensitivity, precision, and specificity for detecting the virus.
  • CRISPR diagnostics are portable and do not require specialized laboratory equipment, enhancing accessibility.

Conclusions:

  • CRISPR-based systems represent a significant advancement in molecular diagnostics for infectious diseases like COVID-19.
  • The speed, accuracy, and portability of CRISPR diagnostics facilitate rapid intervention and disease control.
  • CRISPR technology holds promise for revolutionizing infectious disease surveillance and management globally.