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Experimental immunological intrahepatic cholestasis model.

Y Mizoguchi1, Y Sakagami, K Miyajima

  • 1Third Department of Internal Medicine, Osaka City University Medical School, Japan.

Gastroenterologia Japonica
|February 1, 1988
PubMed
Summary
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Heat-killed Propionibacterium acnes (P. acnes) induced intrahepatic cholestasis in guinea pigs. Subsequent purified protein derivative (PPD) injection exacerbated cholestasis, affecting bile flow and liver enzymes.

Area of Science:

  • Immunology
  • Hepatology
  • Microbiology

Background:

  • Propionibacterium acnes (P. acnes) has been implicated in various inflammatory conditions.
  • Tuberculin hypersensitivity is a well-established immune response.

Purpose of the Study:

  • To investigate the effect of P. acnes and purified protein derivative (PPD) on liver function in a guinea pig model.
  • To elucidate the mechanism of cholestasis induction.

Main Methods:

  • Intravenous injection of heat-killed P. acnes into tuberculin-sensitized guinea pigs.
  • Subsequent intravenous injection of PPD.
  • Monitoring of bile flow, bile acid excretion, serum biochemistry, and liver histology.

Main Results:

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  • P. acnes injection followed by PPD led to significant reductions in bile flow and bile acid excretion.
  • Elevated serum bile acids, cholesterol, alkaline phosphatase (ALK-p), and leucine aminopeptidase (LAP) were observed.
  • Histological findings included bile canaliculi dilatation and microvilli diminution, indicative of intrahepatic cholestasis.

    • Conclusions:

    • The experimental model successfully induced intrahepatic cholestasis.
    • P. acnes appears to facilitate lymphocyte infiltration into the liver, while PPD triggers a cholestatic factor, leading to liver pathology.