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Related Experiment Video

Updated: Nov 19, 2025

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

Cdk5rap3 is essential for intestinal Paneth cell development and maintenance.

Michaela Quintero¹, Siyang Liu¹, Yanhua Xia²

¹Department of Biochemistry & Molecular Biology, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA.

Cell Death & Disease

|January 28, 2021

Summary

Cdk5rap3 protein is essential for Paneth cell development and function in the intestine. Its absence leads to Paneth cell loss and increased susceptibility to colitis, highlighting its role in gut health.

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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

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Published on: November 21, 2015

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Published on: July 13, 2016

Area of Science:

Cell Biology
Gastroenterology
Developmental Biology

Background:

Paneth cells in the intestine are vital for microbiome homeostasis, immune modulation, and stem cell support.
Dysfunctional Paneth cells are implicated in inflammatory bowel disease (IBD) pathogenesis.
The physiological role of Cdk5 activator binding protein Cdk5rap3 (C53/LZAP) is largely unknown.

Purpose of the Study:

To investigate the function of Cdk5rap3 in intestinal epithelial cells and Paneth cell development.
To determine the role of Cdk5rap3 in maintaining intestinal homeostasis and its link to IBD.

Main Methods:

Generation of intestinal epithelial cell (IEC)-specific and Paneth cell-specific Cdk5rap3 knockout mouse models.
Inducible acute deletion of Cdk5rap3 in mature Paneth cells.
Analysis of Paneth cell morphology, gene expression (Gfi1, Sox9), and susceptibility to experimental colitis.

Main Results:

Cdk5rap3 deficiency in IECs caused near-complete Paneth cell loss and heightened colitis susceptibility.
Cdk5rap3 loss downregulated key Paneth cell transcription factors Gfi1 and Sox9.
Acute deletion in mature Paneth cells led to rough endoplasmic reticulum disassembly, abnormal zymogen granules, and cell loss.

Conclusions:

Cdk5rap3 is indispensable for Paneth cell development, differentiation, and maintenance.
Cdk5rap3 plays a critical role in intestinal homeostasis and may be a therapeutic target for IBD.