Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

803
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
803
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

244
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
244
Biofilms01:29

Biofilms

727
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
727
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.8K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.8K
Bacterial Phylum Proteobacteria01:26

Bacterial Phylum Proteobacteria

387
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
387

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized noninferiority field trial evaluating a postmilking teat dip for the prevention of naturally occurring intramammary infections.

Journal of dairy science·2023
Same author

A regional-based newborn hearing screening program: the Emilia-Romagna model after ten years of legislation.

Annali di igiene : medicina preventiva e di comunita·2022
Same author

Executive functions and adaptive behaviour in individuals with Down syndrome.

Journal of intellectual disability research : JIDR·2021
Same author

The predictive value of clinical, radiographic, echocardiographic variables and cardiac biomarkers for assessing risk of the onset of heart failure or cardiac death in dogs with preclinical myxomatous mitral valve disease enrolled in the DELAY study.

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2021
Same author

Multiple retrospective analysis of survival and evaluation of cardiac death predictors in a population of dogs affected by degenerative mitral valve disease in ACVIM class C treated with different therapeutic protocols.

Polish journal of veterinary sciences·2021
Same author

DELay of Appearance of sYmptoms of Canine Degenerative Mitral Valve Disease Treated with Spironolactone and Benazepril: the DELAY Study.

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2020

Related Experiment Video

Updated: Nov 19, 2025

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

26.8K

Pathogenic Microbial Profile and Antibiotic Resistance Associated with Periodontitis.

R Ansiliero1, J M L N Gelinski1, Q L Samistraro1

  • 1Laboratory of Biotechnology, Universidade do Oeste de Santa Catarina, Videira, SC 89560000 Brazil.

Indian Journal of Microbiology
|January 28, 2021
PubMed
Summary

Periodontal disease is linked to a more diverse and numerous bacterial community, including multidrug-resistant strains. Maintaining oral health through prophylactic measures is crucial for disease prevention.

Keywords:
ARDRABacterial communityPeriodontal diseaseβ-lactam resistance

More Related Videos

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.9K
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
11:15

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

11.2K

Related Experiment Videos

Last Updated: Nov 19, 2025

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

26.8K
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.9K
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
11:15

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

11.2K

Area of Science:

  • Microbiology
  • Periodontology
  • Antimicrobial Resistance

Background:

  • Periodontal disease is a significant oral health concern.
  • The global rise in antimicrobial resistance necessitates understanding microbial profiles in disease states.
  • Conventional and molecular techniques are vital for microbial assessment.

Purpose of the Study:

  • To assess pathogenic microbial load in different stages of periodontal disease using ARDRA.
  • To evaluate antibiotic susceptibility profiles of isolated bacterial strains.
  • To investigate the relationship between periodontitis stages and bacterial community diversity.

Main Methods:

  • Phenotyping using conventional microbiological, physiological, and molecular analysis.
  • Amplified Ribosomal DNA Restriction Analysis (ARDRA) technique for bacterial identification.
  • Antibiotic susceptibility testing for isolated strains.

Main Results:

  • Periodontal disease stages showed a significantly more diverse and numerous bacterial community compared to healthy controls.
  • Predominant pathogenic bacteria were Gram-negative, including Citrobacter freundii, Raoutella sp., and Pseudomonas sp.
  • Multidrug resistance to beta-lactam antibiotics was observed in Streptococcus pneumoniae, Raoutella sp., and Enterococcus avium.

Conclusions:

  • A strong correlation exists between periodontitis progression and increased bacterial diversity.
  • Biofilms harboring multidrug-resistant bacteria pose a significant challenge to patient health restoration.
  • Prophylactic oral hygiene measures remain the most effective strategy for preventing periodontal disease.