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Author Correction: Circular RNA 0001273 in exosomes derived from human umbilical cord mesenchymal stem cells (UMSCs)

C-X Li1, J Song, X Li

  • 1Department of Cardiology, Qingdao Central Hospital, Qingdao, China.

European Review for Medical and Pharmacological Sciences
|January 28, 2021
PubMed

Insights

This correction addresses limitations in exosome characterization, including ultrastructure and CD63 immunoblotting. The revised study provides enhanced data on exosomes for medical and pharmacological sciences.

Area of Science:

  • Biomedical Sciences
  • Molecular Biology
  • Cell Biology

Background:

  • Exosomes are crucial nanovesicles involved in intercellular communication.
  • Accurate characterization of exosomes is essential for understanding their biological roles and therapeutic potential.
  • Previous characterization in the original study was identified as limited.

Purpose of the Study:

  • To address criticisms regarding the characterization of exosomes.
  • To enhance the scientific rigor and reproducibility of the study.
  • To provide a more comprehensive analysis of exosome properties.

Main Methods:

  • Re-performance of key assays to validate findings.
  • Transmission Electron Microscopy (TEM) for exosome ultrastructure analysis.
  • Western blotting with improved antibody detection for CD63 expression.

Main Results:

  • Detailed ultrastructural morphology of exosomes was successfully visualized using TEM.
  • A more characteristic CD63 protein band was obtained, confirming exosome marker presence.
  • Spelling and terminological errors, including 'lysate' to 'lysis', were corrected.

Conclusions:

  • The revised study offers a more robust characterization of exosomes.
  • These amendments improve the reliability of the findings presented in the European Review for Medical and Pharmacological Sciences.
  • The enhanced data contributes to a clearer understanding of exosome biology and their applications.

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