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Circular RNA CircMTO1 suppressed proliferation and metastasis of osteosarcoma through miR-630/KLF6 axis

D-Y Liu1, Z Li, K Zhang

  • 1Department of Orthopaedics, Hong Hui Hospital affiliated to Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China. mengyibin_hh@163.com.

European Review for Medical and Pharmacological Sciences
|January 28, 2021
PubMed
Abstract

Insights

CircularMTO1 (circMTO1) suppresses osteosarcoma progression by inhibiting cell proliferation and metastasis. This circular RNA regulates the miR-630/KLF6 axis, offering potential diagnostic and therapeutic strategies for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNAs (circRNAs) are implicated in gene expression regulation and can influence cancer development.
  • Osteosarcoma is a primary bone malignancy with complex genetic underpinnings.

Purpose of the Study:

  • To investigate the expression patterns of circMTO1 in osteosarcoma.
  • To elucidate the molecular mechanism by which circMTO1 affects osteosarcoma progression.

Main Methods:

  • CircRNA microarrays and qRT-PCR were employed to assess circMTO1 expression levels.
  • Functional assays (CCK8, migration, invasion, flow cytometry) evaluated circMTO1's impact on cell behavior.
  • Western blot and recovery experiments explored the underlying molecular pathways.

Main Results:

  • circMTO1 expression was found to be downregulated in osteosarcoma tissues and cell lines.
  • Overexpression of circMTO1 inhibited osteosarcoma cell proliferation, migration, and invasion, while promoting apoptosis.
  • Bioinformatics and experimental data confirmed circMTO1 acts as a sponge for miR-630, regulating the miR-630/KLF6 axis.

Conclusions:

  • circMTO1 functions as a tumor suppressor in osteosarcoma.
  • The circMTO1/miR-630/KLF6 pathway represents a potential therapeutic target for osteosarcoma treatment.
  • circMTO1 holds promise as a diagnostic and therapeutic biomarker for osteosarcoma.

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