Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.4K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.4K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

51.2K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
51.2K
Organization of Genes02:07

Organization of Genes

72.0K
Overview
72.0K
Exon Recombination02:32

Exon Recombination

3.9K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.9K
Complementary DNA01:44

Complementary DNA

30.5K
Overview
30.5K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

12.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SRBench++ : principled benchmarking of symbolic regression with domain-expert interpretation.

IEEE transactions on evolutionary computation : a publication of the IEEE Neural Networks Council·2025
Same author

Invited review: The One Health challenges and opportunities of the H5N1 outbreak in dairy cattle in the United States.

Journal of dairy science·2025
Same author

Quality of life and patient-perceived symptoms in patients with psoriasis undergoing proactive or reactive management with the fixed-dose combination Cal/BD foam: A post-hoc analysis of PSO-LONG.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Response to letter regarding Tobacco smoking.

Journal of internal medicine·2020
Same author

A phase I study of panobinostat and ruxolitinib in patients with primary myelofibrosis (PMF) and post--polycythemia vera/essential thrombocythemia myelofibrosis (post--PV/ET MF).

Leukemia research·2019
Same author

Successful knock-in of Hypertrophic Cardiomyopathy-mutation R723G into the MYH7 gene mimics HCM pathology in pigs.

Scientific reports·2018
Same journal

Development of CypriSSR: a genome-wide, chromosome-level microsatellite database for multiple cyprinidae species.

Database : the journal of biological databases and curation·2026
Same journal

KitBase Expanded: An Integrated Genomic and Phenotypic Resource for 3,268 Fast-Neutron-Irradiated Rice Mutants.

Database : the journal of biological databases and curation·2026
Same journal

PhaLP 2.0: extending the community-oriented phage lysin database with a SUBLYME pipeline for metagenomic discovery.

Database : the journal of biological databases and curation·2026
Same journal

A similarity metric, rubric, and unified hierarchy for biomedical publication types and study designs.

Database : the journal of biological databases and curation·2026
Same journal

GUTAID: a curated database linking gut microbial antigens to autoimmune mechanisms.

Database : the journal of biological databases and curation·2026
Same journal

Rosetta Statements: simplifying FAIR knowledge graph construction with a user-centred approach.

Database : the journal of biological databases and curation·2026
See all related articles

Related Experiment Video

Updated: Nov 19, 2025

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

14.0K

SinEx DB 2.0 update 2020: database for eukaryotic single-exon coding sequences.

R Jorquera1,2, C González1,3, P T L C Clausen4

  • 1Center for Bioinformatics and Genome Biology, Fundacion Ciencia & Vida, Zañartu 1482, Ñuñoa Santiago 7780132, Chile.

Database : the Journal of Biological Databases and Curation
|January 28, 2021
PubMed
Summary
This summary is machine-generated.

Single-exon genes (SEGs) lack introns and are crucial for understanding evolution and diseases. SinEx DB 2.0 now offers comprehensive data on SEGs from 60 eukaryotic genomes, aiding research into their function and links to cancer.

More Related Videos

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.3K
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
08:53

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Published on: June 10, 2017

10.3K

Related Experiment Videos

Last Updated: Nov 19, 2025

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

14.0K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.3K
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
08:53

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Published on: June 10, 2017

10.3K

Area of Science:

  • Genomics
  • Bioinformatics
  • Evolutionary Biology

Background:

  • Single-exon genes (SEGs) are nuclear, protein-coding genes lacking introns in their coding sequences (CDSs).
  • SEGs are implicated in human cancers, neurological, and developmental disorders, often showing tissue-specific expression.
  • Previous databases like SinEx DB provided limited information on SEGs from mammalian genomes.

Purpose of the Study:

  • To present SinEx DB 2.0, a significantly updated and expanded database for single-exon genes.
  • To provide comprehensive data on the occurrence, distribution, and functional predictions of SEGs across diverse eukaryotic genomes.
  • To facilitate research into the evolutionary origins, functional roles, and disease associations of SEGs.

Main Methods:

  • Development of a novel bioinformatics pipeline to accurately identify and disambiguate single-exon isoforms.
  • Integration of DNA and protein sequence data, functional predictions (KOG), and Gene Ontology (GO) functional assignations.
  • Database construction using MySQL Server 5.7, incorporating data from 60 completely sequenced eukaryotic genomes.

Main Results:

  • SinEx DB 2.0 is the largest publicly available database for SEGs, encompassing 60 eukaryotic genomes.
  • The database includes detailed information on SEG occurrence, distribution, and functional predictions.
  • Complete SEG sequence datasets and GO functional annotations are available for download.

Conclusions:

  • SinEx DB 2.0 serves as a valuable resource for studying the evolution and function of SEGs.
  • The expanded dataset aids in understanding the roles of SEGs in various biological processes and diseases.
  • This database supports further research into the links between SEGs and conditions like cancer and neurological disorders.